We demonstrated that the chain-specific C=C positions are inherently encoded in the elution profile of complex lipids in routine reverse-phase chromatography (RPLC). With this foundation, we introduced the LDA C=C Localizer (LC=CL), a computational tool that utilizes a comprehensive database capturing the elution profile of more than 2,400 experimentally validated ω-resolved complex lipid species, including 1,145 newly reported compounds. Here, these retention time databases (RT-DB) are available for download in formats compatible with the LDA software tool (see links on the right side of this page). LC=CL enables accurate, automated C=C position assignment and is adaptable to various chromatographic conditions. Accordingly, C=C position information is now readily accessible for large-scale high-throughput studies with any MS/MS instrumentation and ion activation method.
Select options to download the appropriate RT-DB file alongside reference data for RT-DB mapping.
Installers for LDA2 featuring LC=CL are available for Windows, Linux, and macOS at the LDA download page: